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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Lung delivery of MSCs expressing anti-cancer protein TRAIL visualised with 89Zr-oxine PET-CT

Fig. 2

TRAIL expression and therapeutic function is maintained following radiolabelling. a Western blot analysis on protein extracts 7 days post-radiolabelling shows maintained TRAIL expression. b Viability of two human lung cancer cell lines (NHI-H28 and PC9) was measured using luciferase bioluminescence after treatment with soluble trail at 10 or 50 ng/mL, or co-incubation for 24 h with MSCTRAIL labelled 3 days prior with the indicated amounts, or sham labelled in PBS or PBS with 3% DMSO. Bars show the mean of 4 populations; error bars show SD. c Apoptosis of four cancer cell lines untreated or after incubation with MSCTRAIL (sham labelled in PBS + 3% DMSO) or MSCTRAIL radiolabelled with 89Zr-oxine at 332 kBq/106 cells, measured using Annexin V staining and flow cytometry. Error bars show SD, n = 3. d Representative FACS plots from c, showing apoptosis of H28 cells following incubation with control or radiolabelled MSCTRAIL

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