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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Human ex vivo spinal cord slice culture as a useful model of neural development, lesion, and allogeneic neural cell therapy

Fig. 3

Quantification of glial cells, macrophages and monocytes by flow cytometry. The expression of GFAP (A, F), CD11b+CD45low (B, G), HLA-DR+CD11b+CD45low (C, H), CD11b+CD45high (D, I), and CD11bCD45+ (E, J) was assessed by flow cytometry on slices from in situ and 7, 14, and 21 DIV. The degree of positive cells is presented as percentage of immunoreactive cells out of total cell number. Values are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0,001. Abbreviations: BS-SC, longitudinal brain stem-spinal cord slices; SC, spinal cord cross-sectional slices; DIV, days in vitro; n, number of biological cases analyzed per time point

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