Fig. 6

Human SCI model and experimental allogeneic hfNPC grafting. A Total hOC Score, and B slice area in SC hOCs before (at 7DIV), 1 (7DIV + 1DPI), 7 (7DIV + 7DPI), and 14 (7DIV + 14DPI) DPI; *p < 0.5, ***p < 0.001. A, B n, number of slices (from in total 8 biological cases). C Appearance of lesion at 14 DPI, phase contrast micrograph with a central cavity, D expression of MAP-2 in the center of the lesion, and E GFAP immunoreactivity at injury site. Cell nuclei were stained with Hoechst dye. Arrows point out lesion area. F Flow cytometer quantification of Ki-67⁺, G caspase-3⁺, H GFAP⁺, I Cd11b⁺CD45_low, J HLA-DR⁺Cd11b⁺CD45_low, and K CD68⁺ cells in sham, sham + hfNPC, contusion “SCI,” and contusion “SCI” + hfNPC at 7 and 14 DPI. The percentage of immunoreactive cells out of total cell number is presented. Values are presented as mean ± SEM. *p < 0.05. Abbreviations: DIV, days in vitro; hfNPC, human fetal neural progenitor cells; DPI, days post injury; n, number of biological cases; cSCI, contusion spinal cord injury