Fig. 1
From: Directly induced human Schwann cell precursors as a valuable source of Schwann cells
Generation of iSCPs form human fibroblasts. a Schematic diagram of the iSCP reprogramming process (top). Bright-field images of reprogramming cells (bottom). The iSCP colony (day 18) was coimmunostainned with SCP markers (p75 and SOX10) (bottom). Scale bars = 100 μm. b qPCR analysis of SCP markers (GAP43, SOX10, MPZ, and CDH19) during reprogramming. All the values are relative to human fibroblasts. Mean ± S.E.M. (n = 3–5). c Immunocytochemical analysis of SCP markers (SOX10 and GAP43) in iSCPs (day 18). Scale bars, 100 μm. d Flow cytometry analysis of SCP markers (GAP43, NGFR, SOX10, and MPZ) in iSCPs (day 18). Isotype controls are indicated in blue