Fig. 3
From: Directly induced human Schwann cell precursors as a valuable source of Schwann cells
Differentiation of iSCPs into Schwann cells. a Schematic diagram of the iSC differentiation process (top). Representative phase-contrast images of differentiated iSCs (day 7). Scale bars = 200 μm. b qPCR analysis of SC markers (GFAP, S100b, and PMP22) during differentiation. All the values are relative to day 0 of differentiation. Mean ± S.E.M. (n = 3–5). c Immunoblot analysis of the SC marker S100B during differentiation. Whole cell lysates were electrophoresed by SDS-PAGE, and primary SCs (pSCs) were used as a control. d Immunostaining for the SC markers (SOX10, S100B, and NGFR) after 7 days of differentiation. Cell nuclei were stained with DAPI (blue). Scale bars = 100 μm