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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Knockdown of LAP2α inhibits osteogenic differentiation of human adipose-derived stem cells by activating NF-κB

Fig. 2

Knockdown of LAP2α inhibits osteogenic differentiation of hASCs in vitro. a Left panel: fluorescent micrographs showing the efficiency of lentivirus transfection. Scale bars: 100 μm. Middle and right panel: Validation of LAP2α knockdown effect by qRT-PCR and western blotting, respectively, n = 3. b ALP staining and quantification of cells in the shNC, shLAP2α-1, and shLAP2α-2 groups on day 7 after osteogenic induction, n = 3. c ARS staining and quantification of cells in the shNC, shLAP2α-1, and shLAP2α-2 groups on day 14 after osteogenic induction, n = 3. d Relative mRNA expression of ALP and OCN, as measured by qRT-PCR on day 14 of osteogenic induction. GAPDH was used for normalization, n = 6. Data are shown as the mean ± SD; *P < 0.05 compared with the control group; **P < 0.01 compared with the control group; NS: not significant

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