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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Knockdown of LAP2α inhibits osteogenic differentiation of human adipose-derived stem cells by activating NF-κB

Fig. 6

Knockdown of LAP2α promotes translocation of p65 from the cytoplasm to nucleus. a Relative mRNA expression levels of p65 target genes IL-6, ICAM1, and TRAF1, as measured by qRT-PCR, n = 6. b Proteins levels of p-IκBα, total IκBα, p-p65, and total p65 as measured by western blotting analysis in hASCs expressing shNC, shLAP2α-1, or shLAP2α-2. c Quantification of western blotting analysis, n = 3. d Immunofluorescent confocal microscopy of p65 nuclear translocation and LAP2α expression in hASCs expressing shNC, shLAP2α-1, or shLAP2α-2, treated or not treated with TNF-α for 30 min, scale bars: 50 μm. Data are shown as the mean ± SD; *P < 0.05 compared with the control group; **P < 0.01 compared with the control group; NS: not significant

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