Fig. 1
From: Cutaneous wound healing: canine allogeneic ASC therapy
Differentiation of canine adipose-derived mesenchymal stem cells (ASCs) into osteocyte, chondrocyte, and adipocyte lineages. a Representative photographs. ASCs from the 3rd passage showed spindle-shaped fibroblastic cell morphology (1). After 3 weeks, ASCs differentiated according to the conditioned medium added. Cells underwent von Kossa staining for osteogenic lineage (2), Alcian blue staining for chondrogenic lineage (3), and oil red O staining for adipogenic lineage (4) (bar length = 50 μm). b RT-qPCR analysis of transcription factors in the differentiation of ASCs. Data represent mean fold change values in genes ± SD compared to ASCs. ****A statistically significant difference of p < 0.0001 of each transcription factors representative of a specific lineage with respect to the genes of other lineages. n = 3 different experiments