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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Nonadherent culture method promotes MSC-mediated vascularization in myocardial infarction via miR-519d/VEGFA pathway

Fig. 2

Decreased levels of VEGFA produced by VEGFA knockdown nonAC-hMSCs inhibits the angiogenesis of HUVECs. AC-hMSCs were infected with VEGFA or negative control (NC) lentiviruses and nonAC-hMSCs were infected with shVEGFA or the control lentiviruses. a RT-qPCR and b western blotting were performed to detect mRNA and protein levels of VEGFA, respectively. c ELISA analysis was performed to determine the levels of secreted VEGF. d Capillary-like structure formation of HUVECs cultured in CM from hMSCs with different genotypes was evaluated by tube formation assay. Data are represented as mean ± SD (n = 3 per group). P < 0.05 compared to AC-hMSC-Ctrl or nonAC-hMSC-NC group. P < 0.05 compared to AC-hMSC-Ctrl group

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