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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Nonadherent culture method promotes MSC-mediated vascularization in myocardial infarction via miR-519d/VEGFA pathway

Fig. 3

VEGFA production by VEGFA-overexpressing AC-hMSCs increases angiogenesis and reduces infarction size after MI. a RT-qPCR, b western blotting, and c ELISA were used to assess VEGFA expression and secretion in the heart tissues obtained from LADCA-ligated rats 48 h after transplantation with AC-VEGFA-hMSCs, nonAC-sh-VEGFA-hMSCs, or their corresponding control hMSCs. d Immunohistochemistry analysis for vWF expression in the heart tissues obtained from LADCA-ligated rats 28 days after transplantation with AC-VEGFA-hMSCs, nonAC-sh-VEGFA-h MSCs, or their corresponding control hMSCs. Red arrow indicates the small vessels. e Representative images and quantification of fibrosis area. Data are represented as mean ± SD (n = 8 per group). P < 0.05 compared to AC-hMSC-Ctrl or nonAC-hMSC-NC group. #P < 0.05 compared to MI group. P < 0.05 compared to AC-hMSC-Ctrl group

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