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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Nonadherent culture method promotes MSC-mediated vascularization in myocardial infarction via miR-519d/VEGFA pathway

Fig. 5

VEGFA is a direct target of miR-519d. a The predicted binding site between VEGFA and miR-519d. b RT-qPCR analysis of miR-519d expression in AC-hMSCs and nonAC-hMSCs at 24 h and 72 h. c Luciferase activity in HEK293T cells co-transfected with miR-519d or control mimics and VEGFA-3′ UTR-WT or VEGFA-3′ UTR-Mut plasmids. d–f VEGFA expression and secretion in nonAC-hMSCs transfected with miR-519d or control mimics, and AC-hMSCs transfected with miR-519d or control inhibitors. Data are represented as mean ± SD (n = 3 per group). P < 0.05 compared to miR-Ctrl or anti-miR-Ctrl group. P < 0.05 compared to miR-Ctrl group

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