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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Exosomes derived from human umbilical cord MSCs rejuvenate aged MSCs and enhance their functions for myocardial repair

Fig. 3

ExoUMSCs promotes the biological function and paracrine effects of Old MSCs in vitro. a Mobility of OMSCs with or without treatment of ExoUMSCs was analyzed by the Transwell assay. Migrated cells were visualized by stainings of Hochest for nuclear. Scale bar, 100 μm, and the quantification of the migrated cells in A. b Western blot analysis of Bcl2 and Bax for detection of cell apoptosis. β-actin served as loading control and the quantification of Bcl/Bax ratio in g. Each experiment was repeated three times. c Flow cytometry analysis of apoptosis and necrosis for specified cells after they were stained with Annexin V and Propidium Iodide (PI) and the quantification of cells in either early (Q3) or late (Q2) apoptosis. d Representative images of tube formation assay of HUVECs which were transduced with lentiviral vector carrying gene for GFP and cultured in DMEM alone or conditioned medium of specified cells (OMCs, OMSCsExo, and UMSCs). Scale bar, 200 μm. e Quantification of tube length in A. f Flow cytometry analysis of apoptosis and necrosis in primary mouse cardiomyocytes after they were pretreated with DMEM or specified conditioned medium under hypoxia and serum deprivation condition for 24 h and then stained with Annexin V and PI. g Quantification of Annexin-V-positive cells (Q2 and Q3) in C. *P < 0.05 vs OMSCs; #P < 0.05 vs OMSCsExo

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