Fig. 4

Effects of MSC transplantation on cardiac function after MI. a Representative echocardiographic images of specified mice at day 28 post MI. b Cardiac functions assessed by EF and FS (%) were obtained from echocardiographic images. c Representative images of immunofluorescence staining for ECs using Ab against CD31 (red) to illustrate angiogenesis in the border zone of ischemic hearts 28d post MI. d CD31-positive cells were quantified per high power field (HPF) to calculate the vessel density. e Representative images of immunofluorescence staining for vessel using antibody against α-SMA (green) to illustrate angiogenesis in the border zone of ischemic hearts 28 days post MI. f α-SMA-positive cells were quantified per HPF to calculate the mature vessel density. g Sections of hearts 28 days post MI were stained with Sirus red to illustrate the fibrosis (red) at infarct tissues. h The scar size was quantified and expressed as the ratio of the length of collagen deposited area (red) over perimeter of left ventricle. n = 6 hearts/group. Each experiment was repeated at least three times. *P < 0.05 vs DMEM; ^#P < 0.05 vs OMSCs