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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: hUMSC transplantation restores ovarian function in POI rats by inhibiting autophagy of theca-interstitial cells via the AMPK/mTOR signaling pathway

Fig. 5

hUMSCs co-culture with TICs inhibit autophagy in TICs induced by CDDP. ac TEM Images show normal ultrastructure with few autophagy structures in the Control and CDDP + hUMSCs groups. Within the CDDP group, most autophagy structures are diffusely located within the cytoplasm—black arrow indicates autophagy structure. dh Representative immunofluorescence images of TICs as shown with specific autophagy marker staining of LC3B (green fluorescent) and nuclear DAPI staining (blue fluorescence) within each group. n Quantification of average fluorescent intensities with LC3B staining within each group. im TICs were stained with MDC to determine autophagy levels within each group. o Quantification of average fluorescent intensities with MDC within each group. Data are expressed as the means ± SD, ***P < 0.001. hUMSCs, human umbilical cord-derived mesenchymal stem cells; TICs, theca-interstitial cells; CDDP, cisplatin; DAPI, 4,6-diamino-2-phenyl indole; MDC, dansylcadaverine

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