Fig. 4
From: Impaired autophagy triggered by HDAC9 in mesenchymal stem cells accelerates bone mass loss
Inhibition of autophagy blocked the ability of HDAC9 siRNA to rebalance BMMSC differentiation. To investigate the HDAC9-autophagy axis regulating BMMSC function, BMMSCs were respectively transfected with Nc siRNA and HDAC9 siRNA or co-transfected with HDAC9 siRNA and BECN1 siRNA. a Alizarin Red staining was performed, and osteogenesis-related proteins were analyzed in aged BMMSCs from above three groups. b Oil Red O staining was performed, and adipogenesis-related proteins were analyzed in three groups of cells described above. Scale bars = 100 μm. The data are presented as the means ± SD of each independent experiment performed in triplicate. *P < 0.05, **P < 0.01, one-way analysis of variance (ANOVA)