Fig. 3

Effect of H₂O₂ and probucol on MSC viability and ROS. a H₂O₂ treatment inhibits the viability of MSCs in a concentration-dependent manner. IC50 value was calculated using CCK8 assay in triplicates; subIC50 dose of 200 μM was used for further experiences. b Probucol’s intervention could reverse the injury of H₂O₂ to MSCs, and was statistically significant at the concentration of 150 μM. c Probucol reduces ROS levels in MSCs. Intracellular ROS in the normal group, the oxidative stress group, and the probucol group were measured by flow cytometry before histogram was made. *P < 0.05 and **P < 0.01 indicate significant difference compared with the sham group. ^#P < 0.05 and ^##P < 0.01 indicate significant difference compared with the H₂O₂ group