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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Cellular senescence mediates the detrimental effect of prenatal dexamethasone exposure on postnatal long bone growth in mouse offspring

Fig. 5

Clearance of senescent-cell by treatment with senolytics dasatinib and quercetin (D+Q) rescues the suppressed osteogenesis in female mouse offspring after PDE. a Representative images of senescence associated β-galactosidase (SA-β-Gal) staining, hematoxylin-eosin (H&E) staining, Goldner’s trychrome staining, and Nestin immunofluorescence (green) in femoral metaphysis from 2-week-old female offspring. Pregnant mice were injected subcutaneously with normal saline (control) or dexamethasone (1.2 mg/kg/day) (PDE group) during gestational days (GD) 12–14. Additionally, control pregnant mice were treated with 1% methyl cellulose, and PDE pregnant mice were treated with 1% methyl cellulose (vehicle) or dasatinib (5 mg/kg/day) and quercetin (50 mg/kg/day) by oral gavage during GD 12–14. Femurs of 2-week-old female mouse offspring were harvested for analysis. DAPI stains nuclei blue. Scale bar, 100 μm. b Quantitative analysis of the numbers of SA-β-Gal+ cells per tissue area in femoral metaphysis. n = 6 per group. c Quantitative analysis of the osteoblast numbers per tissue area in femoral metaphysis. n = 6 per group. d Quantitative analysis of the Nestin+ cells per tissue area. n = 5 per group. *P < 0.05, one-way analysis of variance (ANOVA) with Bonferroni post hoc test

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