Fig. 7
Senolytics dasatinib and quercetin (D+Q) rescues the loss of osteoblasts and H type vessels in female mouse offspring after PDE. a Representative images of the immunohistochemistry staining for osteocalcin (Ocn, dark brown stained cells on the surface of trabecular bone) and b the number of Ocn+ cells per mm of bone surface in femoral bone from 4-week-old female mouse offspring. Scale bar, 50 μm. c Representative images of double-immunofluorescence staining for CD31 (green) and Endomucin (Emcn, red) in femoral metaphysis from 4-week-old female offspring. DAPI stains nuclei blue. Scale bar, 30 μm. d Quantification of the relative fluorescence area of CD31+Emcn+ cells per tissue area in femoral metaphysis (CD31+Emcn+ area per mm2). Data are represented as mean ± S.E.M. n = 5 per group, *P < 0.05, one-way analysis of variance (ANOVA) with the Bonferroni post hoc test