DLX5 knock-down inhibited the chondrogenic differentiation in SCAPs. a, b Real-time RT-PCR and Western blot results showed that DLX5 was knocked down in SCAPs. The relative protein levels quantified by densitometry and normalized to GAPDH. c, d Alcian Blue staining and quantitative analysis results show that knock-down of DLX5 reduced chondrogenic differentiation in SCAPs. e–g Real-time RT-PCR results show that knock-down of DLX5 downregulated the expressions of COL2 (e), COL5 (f), and SOX9 (g) in SCAPs. h Alcian Blue and Picro Sirius Red staining results of chondrogenesis induced pellet. Scale bar, 100 μm. GAPDH was used as an internal control. Student’s t test was performed to determine the statistical significance. All error bars represent s.d. (n = 3). **P ≤ 0.01. Consh, control shRNA; DLX5sh, DLX5 shRNA.