Fig. 4

HOXC8 over-expression enhanced the chondrogenic differentiation of SCAPs. a, b Alcian Blue staining and quantitative analysis results showed that HOXC8 over-expression enhanced chondrogenic differentiation in SCAPs. c–e Real-time RT-PCR results showed that HOXC8 over-expression upregulated the expression of COL2 (c), COL5 (d), and SOX9 (e) in SCAPs. f Alcian Blue and Picro Sirius Red staining results of chondrogenesis induced pellet. Scale bar, 100 μm. GAPDH was used as an internal control. Student’s t test was performed to determine the statistical significance. All error bars represent s.d. (n = 3). **P ≤ 0.01