Fig. 5

HOXC8 knock-down inhibited the chondrogenic differentiation of SCAPs. a, b Alcian Blue staining and quantitative analysis results showed that knock-down of HOXC8 reduced chondrogenic differentiation in SCAPs. c–e Real-time RT-PCR results showed that knock-down of HOXC8 downregulated the expressions of COL2 (c), COL5 (d), and SOX9 (e) in SCAPs. f Alcian Blue and Picro Sirius Red staining results of chondrogenesis induced pellet. Scale bar, 100 μm. GAPDH was used as an internal control. Student’s t test was performed to determine the statistical significance. All error bars represent s.d. (n = 3). *P ≤ 0.05. **P ≤ 0.01. Consh, control shRNA; HOXC8sh, HOXC8 shRNA.