Fig. 2

Characterization of the hiPSC-derived cardiomyocytes. a, b CHIR concentration-dependently increased the expression of BRACHYURY (a) and MESP1 (b) in differentiated cells at day 1 and day 3 (t test, *p < 0.05, **p < 0.01, and *** p < 0.001 versus 0 μM or day 1; n = 3). c The hiPSC-induced cardiomyocytes expressed cTNT and α-actinin indicated by IF assay in induced cardiomyocytes from day 21 differentiation. Scale bars, 100 μm (× 400). d Representative plots of flow cytometry displaying high yields of cardiomyocytes derived from hiPSC at day 21. Expression values of all PCR analyses were normalized to the housekeeping gene GAPDH. Data are presented as “mean ± SD’”