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Table 1 Primer sets for qRT-PCR analysis

From: Enrichment differentiation of human induced pluripotent stem cells into sinoatrial node-like cells by combined modulation of BMP, FGF, and RA signaling pathways

Gene

Direction

Sequence (5′–3′)

Product size (bp)

NANOG

Forward

TTTGTGGGCCTGAAGAAAACT

91

Reverse

AGGGCTGTCCTGAATAAGCAG

SOX2

Forward

GCCGAGTGGAAACTTTTGTCG

145

Reverse

GGCAGCGTGTACTTATCCTTCT

OCT4

Forward

CTGGGTTGATCCTCGGACCT

121

Reverse

CCATCGGAGTTGCTCTCCA

BRACHYURY

Forward

CAGTGGCAGTCTCAGGTTAAGAAGGA

122

Reverse

CGCTACTGCAGGTGTGAGCAA

MESP1

Forward

AGCCCAAGTGACAAGGGACAACT

82

Reverse

AAGGAACCACTTCGAAGGTGCTGA

NKX2-5

Forward

CAAGTGTGCGTCTGCCTTT

190

Reverse

CAGCTCTTTCTTTTCGGCTCTA

TNNT2

Forward

TTCACCAAAGATCTGCTCCTCGCT

111

Reverse

TTATTACTGGTGTGGAGTGGGTGTGG

SHOX2

Forward

CAAAGAGGATGCGAAAGGGAT

122

Reverse

AGTGGGTCTCGTCAAAAAGCC

TBX18

Forward

GACGATCTTTCTCCCATCAAGC

124

Reverse

CTATCTTCAGGCGAGTAATCTGC

TBX3

Forward

CCCGGTTCCACATTGTAAGAG

104

Reverse

GTATGCAGTCACAGCGATGAAT

HCN4

Forward

TGGACACCGCTATCAAAGTGG

157

Reverse

CTGCCGAACATCCTTAGGGA

MYL7

Forward

AAGCCATCCTGAGTGCCTTC

127

Reverse

AACATCTGCTCCACCTCAGC

MYL2

Forward

TGAGAGACACCTTTGCTGCC

139

Reverse

GGGTCCGCTCCCTTAAGTTT

MSX2

Forward

CTGGTGAAGCCCTTCGAGAC

133

Reverse

ATATGTCCTCCTACTCCTGCCC

TBX2

Forward

TACGCTTGTACGAGGAGCAC

157

Reverse

CACGACTTCTCCTCAGCTCG

ISL1

Forward

TCCCTATGTGTTGGTTGCGG

194

Reverse

CATTTGATCCCGTACAACCTGA

Cx30.2

Forward

CCGAGAACCTTGCCTTGGTA

98

Reverse

GACAGAAACCGCTGACTCCA

Cx43

Forward

TACCAAACAGCAGCGGAGTT

139

Reverse

TGGGCACCACTCTTTTGCTT

Cx40

Forward

AGAGTGTGAAGAAGCCCACG

70

Reverse

AACAGATGCCAAAACTTCTGCT

HCN1

Forward

GCCATGCTGAGCAAGTTGAG

178

Reverse

TCAGCAGGCAAATCTCTCCA

KCND2

Forward

GGGTCTTCGGCTAGCAAGTT

91

Reverse

GCACCATGTCACCATACCCTA

KCNK2

Forward

TGGAACAAGACTCCTTGCTGG

150

Reverse

CTGCCGAACATCCTTAGGGA

KCNN4

Forward

CCGAGAGGCAGGCTGTTAAT

81

Reverse

AGCCGATGGTCAGGAATGTG

KCNJ5

Forward

CCCACAACAGGGAGAGGTTC

170

Reverse

AGCCATAGCTGGGATGTTGTT

KCNQ1

Forward

GGGCCGCGTCTACAACTTC

169

Reverse

CAGCACGATCTCCATCCAGAA

CACNA1A

Forward

GTCTGGGGAAGAAGTGTCCG

151

Reverse

GCTCCTCCCTTGGCAATCTT

CACNB1

Forward

CCAGTGCCAAACAGAAGCAG

181

Reverse

CGAGTGATGGAGATCCTGCC

CACNA1C

Forward

GACGTGCTGTACTGGGTCAA

125

Reverse

AACTCTCCGCTAAGCACACC

SCN5A

Forward

GGAGGAGTCCAGCAAGCAG

194

Reverse

AACTGTCCTCTGGGGTCTCA

SCN3B

Forward

ACGCATTCTGTAGCCCAGAC

156

Reverse

CTTCCAAGGCTCTCGCCTC