Fig. 6From: Placenta-derived multipotent mesenchymal stromal cells: a promising potential cell-based therapy for canine inflammatory brain diseaseInhibition of lymphocyte proliferation by canine ASCs and PMSCs occurs through different mechanisms. Cell cycle analysis was performed using peripheral blood mononuclear cells (PBMCs) and BrdU 5-bromo-2′-deoxyuridine and 7-aminoactinomycin D was measured. Unstimulated PBMCs and mitogen (ConA) activated PBMCs were used as controls. a Canine PMSCs inhibit lymphocyte proliferation by inducing apoptosis. Alternatively, canine ASCs caused cell cycle arrest which is demonstrated by PBMCs accumulating in G0/G1 (b) and hindering cells from entering G2/M (c) or DNA synthesis (S phase) (d). Representative images of cell cycle flow scatter plots and gating strategies for leukocyte DNA content (7-AAD) and proliferation via BrdU incorporation of PBMC controls (e, f) and co-incubations with canine ASCs (e) and PMSCs (f) are shown. BrdU, 5-bromo-2′-deoxyuridine and 7-aminoactinomycin D; ConA, concanavalin A; LSA, leukocyte suppression assay; MSC, mesenchymal stem cellBack to article page