Fig. 2

Ddx56 ΔC-ter overexpression impairs mESC proliferation. a Schematic diagram of full-length Ddx56 and various domain truncations. b mESCs were cultured with Dox (1 μg/mL) or without Dox for 4 days and visualized by phase-contrast microscopy. EGFP iOE mESCs served as a negative control. × 4, objective. Bar, 800 μm. c Colony size from b was quantified by pixels per inch (PPI), 100 colonies were calculated. Error bars indicate SD (n = 3), ***p < 0.001. d Cells were cultured with (+Dox) or without (−Dox) doxycycline for 2 days, then subjected to polysome fractionation for ribosome. e Cells were treated with doxycycline 24 h after seeding and were counted after 2, 4, and 6 days. Error bars indicate SD (n = 3), ***p < 0.001. f Cell cycle was measured by flow cytometry with propidium iodide staining (PI) after treatment with doxycycline for 6 days. Error bars indicate SD (n = 3), *p < 0.05, **p < 0.01