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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Quantification of the CM-Dil-labeled human umbilical cord mesenchymal stem cells migrated to the dual injured uterus in SD rat

Fig. 1

Human umbilical cord mesenchymal stem cells (hUC-MSCs) characterization and CM-Dil-labeled hUC-MSCs (ad). After 1–2 days of inoculation, the tissue masses began to adhere to the wall, and 6–7 days after inoculation, the cells could be seen climbing out of the tissue mass, mostly in fusiform shape; the cell fusion reached more than 80% in about 2 weeks, showing a long and fibrous mesenchymal-like morphology, and then following cell passage (a). The flow cytometry indicated that CD29, CD73, CD90, and CD105 were positive markers; CD34, CD45, and HLA-DR were the negative markers of hUC-MSCs at passage 3 (b). Ninety percent of CM-DiI-labeled cells were observed under a fluorescence microscope (c). The stained cells presented the same proliferation rate with hUC-MSCs at passage 3 (d)

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