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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Quantification of the CM-Dil-labeled human umbilical cord mesenchymal stem cells migrated to the dual injured uterus in SD rat

Fig. 3

The red fluorescent cells were observed in the rat uterus regions (ae). The uterus tissue consists of endometrium epithelium, stroma, deep myometrium (DM), superficial myometrium (SM), and serosa, from the inside to the outside (a). CM-Dil-Labeled hUC-MSCs were detected in the serosa and DM (1), in the SM (2), in the stroma (3), but not found in the epithelium of endometrium and gland (4) from the model with hUC group (b). The fluorescent cells were not detected in the same regions of the other groups (c). The number of fluorescent cells in different regions of the uterus in the model with the hUC group was counted. CM-Dil-labeled cells in the stroma region were significantly higher than those in the seroma (*p = 0.000), in the epithelium (*p = 0.000), and in the SM (*p = 0.005). However, there were no differences between the stroma and the DM (p = 0.93), or the DM and the SM (p = 0.33) (d). Detection of the CXCR4 expression around the damaged uterine tissues in terms of protein level. The protein level of CXCR4 in the model with the hUC group was significantly higher than those in the other groups. *p < 0.05 was considered to be a significant difference (e)

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