Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Prophylactic therapy with human amniotic fluid stem cells improved survival in a rat model of lipopolysaccharide-induced neonatal sepsis through immunomodulation via aggregates with peritoneal macrophages

Fig. 2

hAFSCs attenuated inflammation in the brain, lung, liver, and kidney after LPS challenge. Histology of the brain, lung, and liver tissues from the control (n = 6), LPS (n = 8), and hAFSCs+LPS (n =8) 48 h after LPS exposure. a Representative image of brains. GFAP staining, upper row (scale bars, 50 μm); and Iba-1 staining, lower row (scale bars, 50 μm). The graphs showed the number of GFAP-positive cells/HPF and Iba-1-positive cells/HPF in each group. b Representative image of the lungs. H&E staining, upper row (scale bars, 100 μm); MPO staining, middle row (scale bars, 50 μm); and Iba-1 staining, lower row (scale bars, 50 μm). Radial alveolar counts and mean linear intercepts were performed by averaging seven measurements per rat. The other graphs in the lower row show the percentage of MPO-positive cells/HPF and the number of Iba-1-positive cells/HPF in each group. c Representative image of livers. H&E staining, the first row (scale bars, 100 μm); MPO staining, the second row (scale bars, 50 μm); Iba-1 staining, the third row (scale bars, 50 μm); and PAS staining, the last row (scale bars, 200 μm). The graphs in the upper row show the percentage of MPO-positive cells/HPF and the number of Iba-1-positive cells/HPF in each group. The graphs in the lower row show the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum in each group 48 h after LPS exposure. Results are presented as mean ± SEM. *p < 0.05

Back to article page