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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Human umbilical cord multipotent mesenchymal stromal cells alleviate acute ischemia-reperfusion injury of spermatogenic cells via reducing inflammatory response and oxidative stress

Fig. 1

hUC-MSC alleviated spermatogenic cells injury during testicular torsion and detorsion. a H&E staining of rat testicular tissues at day 1 (D1), day 3 (D3), day 7 (D7), and day 15 (D15) after detorsion. The testes performed torsion and detorsion without hUC-MSC grafts were used as control. The normal group was untreated animals. Scale bars, 100 μm. b Johnsen’s score was evaluated at indicated day after hUC-MSC treatment. c Staining with PNA. Scale bars, 200 μm. d Quantification of seminiferous tubules containing PNA-positive cells. Ten representative sections of the pattern of testes were counted. At least three rats were used in every group. Data were represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. e Immunostaining of rat seminiferous tubules at indicated day after torsion with proliferation marker Ki67. Scale bars, 50 μm. f Immunostaining of rat seminiferous tubules at indicated day after torsion with germ cell marker DDX4. Scale bars, 50 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512