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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human umbilical cord multipotent mesenchymal stromal cells alleviate acute ischemia-reperfusion injury of spermatogenic cells via reducing inflammatory response and oxidative stress

Fig. 2

hUC-MSC injection reduced inflammatory factor production, Selectin-E expression, and neutrophil infiltration of testicular tissues in rats subjected I/R injury. a Real-time PCR analysis of mRNA expression of TNF-α and IL-1β in testicular tissues. The level of mRNA in control testes without hUC-MSC grafts was set as 1. Data was collected from at least 3 rats. b Relative mRNA expression of Selectin-E in testicular tissues. c Immunofluorescence staining of testicular tissues of rats with Selectin-E antibody. Scale bars, 50 μm. d Quantification of fluorescence density of Selectin-E in every seminiferous tubule. At least 10 representative sections of testes were counted. At least three rats were used in every group. Data were represented as mean ± SEM. E. Immunofluorescence staining of testicular tissues with MPO antibody. Scale bars, 50 μm. f Quantification of fluorescence density of MPO of every seminiferous tubule. At least 10 representative sections of testes were counted

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Stem Cell Research & Therapy

ISSN: 1757-6512