Fig. 5

hUC-MSC-CM inhibits inflammatory factors production and inflammatory pathway in HUVECs. HUVECs stimulated by 10 ng/ml TNF-α were cultured with or without hUC-MSC-CM. Twenty-four hours later, the cells were harvested for RNA extract. a Real-time PCR analysis of mRNA levels of TNF-α, IL-1β, and Selectin-E of HUVECs. The level of mRNA in HUVECs was set as 1. Data was collected from at least 3 separated experiments. b ELISA analysis of IL-1β concentration in the supernatant of HUVECs. c Western blot analysis of Selectin-E expression of HUVECs with Selectin-E and β-actin antibodies. d Western blot analysis of P65 and P38 activation of HUVECs with antibodies against P-P65, P65, P-P38, P38, and β-actin. CM represents hUC-MSC-CM