Fig. 1

Identification of METTL1-regulated genes by RNA-seq analysis in hiPSCs. HiPSCs were transduced with shRNAs-METTL1, and pooled clones were selected. a Volcano plot illustrating differentially expressed genes (DEGs) between control and METTL1 knockdown (KD) hiPSCs. Genes upregulated and downregulated are shown in yellow and blue, respectively. Values are presented as the log2 of tag counts. b A total of 37,404 genes were differentially expressed, of which 2301 (6%) were upregulated and 4125 (11%) were downregulated in METTL1-KD hiPSCs. c Heatmap showing DEGs involved in stem cells pluripotency pathways. Each lane corresponds to an independent biological sample. Scale bar: log2 FPKM. d qRT-PCR analysis assessing the mRNA levels of genes involved in pluripotency, in control and METTL1-KD hiPSCs. e KEGG pathway analysis of genes downregulated in METTL1-KD hiPSCs. f Gene ontology (GO) functional clustering of genes downregulated in METTL1-KD hiPSCs; the top 4 most significant biological processes are shown. g qRT-PCR validation analysis shows the mRNA expression fold change of neuroectoderm-, endoderm- and mesoderm-specific genes in METTL1-KD vs. control hiPSCs. Data are presented as the mean ± SEM from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001