Fig. 2

METTL1 is essential for hiPSC self-renewal and pluripotency. Different METTL1-KD hiPSC single-cell clones were selected, and two clones were used for further analyses. a qRT-PCR analysis of METTL1 mRNA expression in control and METTL1-KD hiPSC. b Western blot of the control and METTL1-KD samples with indicated antibodies. c Cell proliferation in control and METTL1-KD hiPSC. The cell numbers were quantified after 3, 5, and 7 days of culture; n = 3. d, e Cell cycle analysis in METTL1-KD and control hiPSC. f, g Colony formation assay of control and METTL1-KD hiPSC. Colony size and numbers were measured at day 7. f Representative images of the colonies. Scale bar, 100 μm. g Colony diameter and number quantification. h, i Alkaline phosphatase (AP) staining of control and METTL1-KD colonies. h Representative images; scale bar, 100 μm. i Percentage of AP positive colonies. The data are presented as mean ± SEM; n = 3; *P < 0.05; **P < 0.01; ***P < 0.001