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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: METTL1-mediated m7G methylation maintains pluripotency in human stem cells and limits mesoderm differentiation and vascular development

Fig. 4

METTL1 silencing accelerates embryonic body formation and increases the expression of angiogenesis-related genes. a Schematic diagram showing the EB spheroid formation process. Cells were seeded on ultralow attachment 96-well spheroid microplates to induce EB formation. b Representative images and quantification of EB formation in control and METTL1-KD hiPSCs on day 6. Scale bar, 50 μm. c qRT-PCR analysis assessing the mRNA levels in control and METTL1-KD EBs. d, e Gene ontology analysis showing functional enrichment in biological process in genes downregulated and upregulated in METTL1-KD EBs. f qRT-PCR analysis assessing the mRNA levels of angiogenesis- and vasculogenesis-related genes in control and METTL1-KD EBs. g GSEA showing enrichment of the mesoderm and cardiovascular development gene signatures. Y-axis indicates enrichment score (ES), while the X-axis indicates positively and negatively correlated gene sets. h KEGG pathway analysis of genes upregulated in METTL1-KD EBs. Data are presented as mean ± SEM; n = 3; *P < 0.05, **P < 0.01; ***P < 0.001

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