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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: METTL1-mediated m7G methylation maintains pluripotency in human stem cells and limits mesoderm differentiation and vascular development

Fig. 5

METTL1 knockdown accelerates teratoma development and angiogenesis in nude mice. a Teratomas formed 6 weeks post-injection in BALB/C nude mice. b Teratoma weight and volume were quantified; n = 5. c, d Immunohistochemical analysis of teratomas from METTL1-KD or control hiPSCs. Scale bar, 2.5 mm and 250 μm, respectively. Quantification of cell number is shown. e Quantification of CD31, vWF, VEGFR2, and SM22α mRNA levels in teratomas from METTL1-KD and control hiPSCs; n = 7 for tumor and control samples. f Representative western blot images for CD31, METTL1, and SM22α protein levels in teratomas from METTL1-KD hiPSCs and control hiPSCs; n = 3. Data are presented as mean ± SEM; *P < 0.05; ***P < 0.001; ****P < 0.0001

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