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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Osteocyte-derived exosomes induced by mechanical strain promote human periodontal ligament stem cell proliferation and osteogenic differentiation via the miR-181b-5p/PTEN/AKT signaling pathway

Fig. 3

Effects of Exosome-MS on osteogenic differentiation of HPDLSCs. ALP and Alizarin red staining were used to evaluate the osteogenic differentiation of HPDLSCs in different treatment groups. a ALP staining (scale bar 100 μm). b Alizarin red staining (scale bar 200 μm). c Relative ALP-positive area as calculated by ImageJ. d Relative Alizarin red-positive area as calculated by ImageJ. e, f The expression levels of BMP2 and Runx2 in different groups were verified using real-time PCR (*P < 0.05, **P < 0.01, and ***P < 0.001)

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