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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Extracellular vesicles from deciduous pulp stem cells recover bone loss by regulating telomerase activity in an osteoporosis mouse model

Fig. 1

Characterization of SHED-derived extracellular vesicles (SHED-EVs). SHED-EVs were isolated from SHED-conditioned medium harvested from 3-day cultures. a Representative histogram of the particle size of SHED-EVs by nanotracking particle analysis. b Representative histograms of the expression of CD9, CD63, CD81, and CD90 in SHED-EVs by flow cytometric (FCM) analysis. PE, R-phycoerythrin. Areas filed with red, target antibody-stained histograms; solid lines, isotype-matched control-stained histograms. c Representative Western blotting images of the expression of CD63, CD81, and calnexin (CANX) in SHED and SHED-EVs. d Representative histograms of the small RNA and microRNA (miRNA) content within SHED-EVs. e, f SHED-EVs were treated with RNase A (5 U/mL; RNase) or PBS (MOCK) for 30 min. Representative histograms of the small RNA and miRNA content in SHED-EVs (e) and particle concentration per particle size of SHED-EVs (f)

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Stem Cell Research & Therapy

ISSN: 1757-6512