Fig. 3

Systemic SHED-EV infusion rescued the properties and functions of mouse bone marrow mesenchymal stem cells (mBMMSCs) in recipient OVX mice. a The ratio of the expression of Tert in mBMMSCs by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The data are shown as a ratio to the expression in the mBMMSCs derived from sham-operated mouse (Sham = 1). b Telomerase activity in mBMMSCs represented by their Ct values using the RQ-TRAP assay. 293T, HEK 293T cells; 293T H.I., heat-inactivated HEK 293T cells. c Number of adherent colonies and proportion of bromodeoxyuridine-positive (BrdU⁺) cells as detected by the CFU-F and BrdU incorporate assays. d Proportion of cells positive for each surface marker in mBMMSCs as determined by FCM analysis. a–d Sham, sham-operated group; OVX, PBS-infused OVX group; MOCK-EV, MOCK-treated SHED-EV-infused OVX group; RNase-EV, RNase-retreated SHED-EV-infused OVX group. n = 7 for all groups. Comparisons between two groups were analyzed by an independent two-tailed Student’s t test. Multiple group comparisons were performed by a one-way analysis of variance followed by Tukey’s post hoc test.*P < 0.05, **P < 0.01, ***P < 0.005, ns no significance. Graph bars represent mean ± SEM