Fig. 6

Effect of MAPC-CM on inflammation in excisional wounds. Representative images of immunofluorescent NIMP-R14 positive neutrophils (green) counterstained with DAPI (blue) in wounds at days 3, 7 and 14 post-injury (a). Quantification of neutrophil numbers in the wound bed normalised to wound area (b). Representative images of immunofluorescent macrophages by F4/80 (green) and YM-1 (red) staining counterstained with DAPI (blue) in wounds (c). Quantification of total macrophage number (F4/80+) in the wound bed normalised to wound area (d). Quantification of M2 macrophages (F4/80+, YM-1+) as a percentage of total macrophages in the wound bed normalised to the wound area. Images captured at 20× objective. n = 8. All data is represented as mean ± SEM. Statistical significance calculated by two-way ANOVA followed by Bonferroni post-tests, where * = p < 0.05, ** = p < 0.01, *** = p < 0.001, ns = non-significant