Fig. 8

Effect of MAPC-CM on total collagen production and collagens I and III expression in excisional wounds. Representative images of collagen detection (green) by Masson’s trichrome staining Images captured at 10× objective (a). Quantification of total collagen levels in wound bed normalised to the normal skin collagen (b). Representative images of immunofluorescent detection of collagen I and collagen III (green) counterstained with DAPI. Images captured at 20× objective (c). Quantification of collagen I expression as mean grey intensity within the wound bed and normalised to the normal skin collagen I level (d). Quantification of collagen III expression as mean grey intensity within the wound bed and normalised to the normal skin collagen III level (e). Values are mean ± SEM of 3 independent experiments. n = 6. Statistical significance calculated by two-way ANOVA followed by Bonferroni post-tests, where * = p < 0.05, ** = p < 0.01, *** = p < 0.001, ns = non-significant