Table 1 Primers used for qPCR of human genes
Gene | Sequence (5′ to 3′) | Amplicon size (bp) |
|---|---|---|
ACAN | F: TCGAGGACAGCGAGGCC R: TCGAGGGTGTAGCGTGTAGAGA | 85 |
COL1A1 | F: CAGCCGCTTCACCTACAGC R: TTTTGTATTCAATCACTGTCTTGCC | 83 |
COL2A1 | F: GGCAATAGCAGGTTCACGTACA R: CGATAACAGTCTTGCCCCACTT | 79 |
RPLP0 | F: TGTGGGCTCCAAGCAGATGCA R: GCAGCAGTTTCTCCAGAGCTGGG | 137 |
COL10A1 | F: ACTCCCAGCACGCAGAATCCA R: TGGGCCTTTTATGCCTGTGGGC | 132 |
RUNX2 | F: GGAGTGGACGAGGCAAGAGTTT R: AGCTTCTGTCTGTGCCTTCTGG | 133 |
SOX9 | F: ACTCCTCCTCCGGCATGAG R: GCTGCACGTCGGTTTTGG | 102 |
IHH | F: ATGAAGGCAAGATCGCTCG R: GATAGCCAGCGAGTTCAGG | 149 |
BGLAP/PB-OST | F: GAAGCCCAGCGGTGCA R: CACTACCTCGCTGCCCTCC | 70 |