Table 1 Primers used for qPCR of human genes
| Gene | Sequence (5′ to 3′) | Amplicon size (bp) |
|---|---|---|
| ACAN |
F: TCGAGGACAGCGAGGCC R: TCGAGGGTGTAGCGTGTAGAGA | 85 |
| COL1A1 |
F: CAGCCGCTTCACCTACAGC R: TTTTGTATTCAATCACTGTCTTGCC | 83 |
| COL2A1 |
F: GGCAATAGCAGGTTCACGTACA R: CGATAACAGTCTTGCCCCACTT | 79 |
| RPLP0 |
F: TGTGGGCTCCAAGCAGATGCA R: GCAGCAGTTTCTCCAGAGCTGGG | 137 |
| COL10A1 |
F: ACTCCCAGCACGCAGAATCCA R: TGGGCCTTTTATGCCTGTGGGC | 132 |
| RUNX2 |
F: GGAGTGGACGAGGCAAGAGTTT R: AGCTTCTGTCTGTGCCTTCTGG | 133 |
| SOX9 |
F: ACTCCTCCTCCGGCATGAG R: GCTGCACGTCGGTTTTGG | 102 |
| IHH |
F: ATGAAGGCAAGATCGCTCG R: GATAGCCAGCGAGTTCAGG | 149 |
| BGLAP/PB-OST |
F: GAAGCCCAGCGGTGCA R: CACTACCTCGCTGCCCTCC | 70 |