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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Nanosecond pulsed electric fields enhance mesenchymal stem cells differentiation via DNMT1-regulated OCT4/NANOG gene expression

Fig. 3

DNMT1 responds to nsPEFs with a short window of 3 days. a Western blot for DNMT1 protein expression level of pMSCs and hMSCs at 2 h after stimulation by nsPEFs. (3 batches of studies were tested with 3 biological donors, values are mean ± SEM from one representative batch with 5 technical repeats, one-way ANOVA, *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, NS, p > 0.05) b qRT-PCR for the expression of DNMT1 of pMSCs and hMSCs at 2 h after stimulation by nsPEFs. (3 batches of studies were tested with 3 biological donors, values are mean ± SEM from one representative batch with 5 technical repeats, one-way ANOVA, *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, NS, p > 0.05) c The global DNA methylation level at 2 h after stimulation by nsPEFs. (n = 6, one-way ANOVA, ****p < 0.0001, NS = 0.1515, ***p = 0.0002) d Protein quantification for the expression of DNMT1 at 2, 12, 24, and 72 h after stimulation by nsPEFs. (3 batches of studies were tested with 3 biological donors, values are mean ± SEM from one representative batch with 5 technical repeats, one-way ANOVA, *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, NS, p > 0.05)

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