Fig. 4

Overexpression of DNMT1 blocks demethylation caused by nsPEFs. a Schematic of MSCs stimulated by nsPEFs with overexpression of DNMT1. b Western blot for DNMT1 after pre-treated with nsPEFs with overexpression of GFP or DNMT1. (3 batches of studies were tested with 3 biological donors, values are mean ± SEM from one representative batch with 5 technical repeats, one-way ANOVA, *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, NS, p > 0.05) c Quantitative RT-PCR for the expressions of OCT4 and NANOG at 2 h after stimulation by nsPEFs. (3 batches of studies were tested with 3 biological donors, values are mean ± SEM from one representative batch with 5 technical repeats, one-way ANOVA, *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, NS, p > 0.05) d Percentage of CpG demethylation for OCT4 and NANOG promoter. (Values are mean ± SD, n = 3, one-way ANOVA, *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, NS, p > 0.05)