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Table 1 Quantification of histological staining intensity of MSCs preconditioned with five parameters of nsPEFs and differentiated to osteogenic, adipogenic, and chondrogenic lineage for 14 days

From: Nanosecond pulsed electric fields enhance mesenchymal stem cells differentiation via DNMT1-regulated OCT4/NANOG gene expression

Fold P value

10 ns, 20 kV/cm

60 ns, 5 kV/cm

60 ns, 10 kV/cm

60 ns, 20 kV/cm

100 ns, 10 kV/cm

Osteogenic

1.58 ± 0.05****

1.34 ± 0.07*

1.15 ± 0.07 NS

1.20 ± 0.09 NS

1.48 ± 0.06***

Adipogenic

1.72 ± 0.03****

1.21 ± 0.10 NS

1.34 ± 0.10**

1.46 ± 0.10***

1.83 ± 0.03****

Chondrogenic

1.46 ± 0.05***

1.96 ± 0.17****

1.30 ± 0.08 NS

1.76 ± 0.10***

1.45 ± 0.03****

  1. Three batches of studies were tested with 3 biological donors, values are mean ± SEM from one representative batch with 5 technical repeats, one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and NS, p > 0.05
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Stem Cell Research & Therapy

ISSN: 1757-6512