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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: RETRACTED ARTICLE: Overexpression of TGFβ1 in murine mesenchymal stem cells improves lung inflammation by impacting the Th17/Treg balance in LPS-induced ARDS mice

Fig. 1

Measurement of TGFβ1 expression in mMSCs after lentiviral vector transduction. a mBM-MSC, mBM-MSC-NC, and mBM-MSC-TGFβ1 were cultured for 20 passages and observed by light microscopy (top) and fluorescence microscopy with green fluorescent protein (bottom), × 200. b The percentage of GFP-positive cells was analysed by flow cytometry at passage 20 after transduction. c Evaluation of TGFβ1 mRNA expression in mMSCs after transduction (n = 3; *p < 0.0001 vs. mBM-MSC-NC). d Evaluation of secreted TGFβ1 in the culture medium of mMSCs after transduction by ELISA (n = 3; *p < 0.0001 vs. mBM-MSC-NCs). e Detection by western blot analysis of TGFβ1 protein levels in mMSCs after transduction (n = 3; *p < 0.0001 vs. mBM-MSC-NCs). mMSCs, mouse mesenchymal stem cells; NC, normal control

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Stem Cell Research & Therapy

ISSN: 1757-6512