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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Transcriptional profiling reveals altered biological characteristics of chorionic stem cells from women with gestational diabetes

Fig. 2

Validation of gene expression and altered biological functions in GDM-CMSCs through real-time PCR and in vitro migration assays. a Upregulation in genes associated with cell migration/cellular assembly (CD24, FLNB, AQP1) and wound healing (EDN1, HBEGF) in GDM-CMSCs validated by real-time PCR with 10 Healthy- and 11 GDM-CMSCs samples. Gene expression levels were normalised to GAPDH and presented as fold change by comparing G-CMSCs to H-CMSCs using 2^-(ΔΔCt) method. b Motility of cell was evaluated by Transwell migration assay. Representative images of migrated Healthy-CMSCs and GDM-CMSCs stained with crystal violet after 8 and 24 h of migration period. Scale bar 150 μm. c Cell migration ability was calculated by counting migrated cells per field by ImageJ. Data represent 6 independent experiments in triplicate. d The graph indicates wound closure percentages, and the images below are representative images of wound healing assay. Healthy-CMSCs and GDM-CMSCs migrated into the middle wound field after 12 h. The percentage of wound closure was calculated by measuring the reduced wound area after 6 h and 12 h by ImageJ. Data were obtained from 6 independent experiments. Scale bar 150 μm. e Significant upregulation of cardiogenic genes in GDM-CMSCs. Validation of NKX2.5, NOG, and PDGFA expression in healthy and GDM samples was examined by real-time PCR. The expression level of each gene was normalised to GAPDH. f Significant downregulation of vasculogenic genes in GDM-CMSCs. RASIP1, CXCL12, and RSPO3 expressions were validated by real-time PCR. The expression level of each gene was normalised to GAPDH. All error bars in this figure are presented as mean ± SEM. Student’s t test was used for statistical significance, *p < 0.05, **p < 0.01, ***p < 0.001

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