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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Hepatocyte growth factor (HGF) and stem cell factor (SCF) maintained the stemness of human bone marrow mesenchymal stem cells (hBMSCs) during long-term expansion by preserving mitochondrial function via the PI3K/AKT, ERK1/2, and STAT3 signaling pathways

Fig. 3

hBMSCs cultured in SHED-CM had enhanced osteogenic differentiation potential during long-term expansion. Results of the analysis on the expression of osteogenic-related proteins in passage 3 (P3) and passage 8 (P8) hBMSCs cultured in DMEM, SHED-CM (P8-SHED-CM), and hBMSCs-CM (P8-hBMSCs-CM). a Relative mRNA expression levels of Runx2, BSP, and ALP per treatment group after osteogenic induction for 7 days. b Western blot results and relative expression levels of Runx2 and BSP proteins per treatment group after osteogenic induction for 7 days. c Alizarin Red staining (ARS) after osteogenic induction for 14 days and the corresponding ARS relative absorbance OD of hBMSCs at 562 nm. ALP staining of hBMSCs after osteogenic induction for 14 days. Plus sign indicates with osteogenic induction. *P < 0.05, **P < 0.01, ***P < 0.001

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