Fig. 7

HGF and SCF preserved the mitochondrial function in hBMSCs via the PI3K/AKT, ERK1/2, and STAT3 signaling pathway. a Western blot results of the expression of mitochondrial-relative proteins (Mfn1, Mfn2, SOD2, and Catalase) and the PI3K/AKT, Erk1/2, and STAT3 signaling pathway-related proteins in passage 3 (P3) and passage 8 (P8) hBMSCs cultured in SHED-CM (P8-SHED-CM) and treated with 100 ng/ml (P8-HGF 100), 10 ng/ml SCF (P8-SCF 10), and the combination of 100 ng/ml HGF and 10 ng/ml SCF (P8-H+S). b Relative expression levels of the protein showed in the Western blot. ns, not significant. c Western blot results of the expression of mitochondrial oxidative stress-related proteins, Catalase and SOD2, and the PI3K/AKT signaling pathway-related proteins in P3 hBMSCs treated with 100 ng/ml HGF, 10 μM LY294002 plus 100 ng/ml HGF, 10 ng/ml SCF, and 10 μM LY294002 plus 10 ng/ml SCF. d Western blot results of the expression of mitochondrial oxidative stress-related proteins, Catalase and SOD2, and the PI3K/AKT signaling pathway-related proteins in P3 hBMSCs treated with 100 ng/ml HGF, 10 μM U0126 plus 100 ng/ml HGF, 10 ng/ml SCF, and 10 μM U0126 plus 10 ng/ml SCF. e Western blot results of the expression of mitochondrial oxidative stress-related proteins, Catalase and SOD2, and the PI3K/AKT signaling pathway-related proteins in P3 hBMSCs treated with 100 ng/ml HGF, 5 μM Stattic plus 100 ng/ml HGF, 10 ng/ml SCF, and 10 μM Stattic plus 10 ng/ml SCF. *P < 0.05, **P < 0.01, ***P < 0.001