Fig. 4

miR-200a-3p regulates MMP-3 and MMP-13 expression in EfS-treated BMSCs. a mRNA expressions of MMP-3 and MMP-13 were increased on stimulation of EfS in a dose-dependent manner with 24 h incubation. β-actin was used as internal control. b Protein expressions of MMP-3 were upregulated with increasing concentration of EfS after 48 h incubation. c Protein expressions of MMP-13 were upregulated with increasing concentration of EfS after 48 h incubation. In b and c, the levels of β-actin were used as internal control. d MMP-3 protein levels were detected by western blot in BMSCs treated with EfS (10%) together with miR-200a-3p mimics (100 nM) or inhibitors (100 nM). e MMP-13 protein levels were detected as in d. *p < 0.05; NS, not significant