Fig. 6

miR-200a-3p affects the expression of MMP-3/MMP-13 through regulating NFκB activity. a Increase of the NFκB activity by E. faecalis supernatants. The NFκB activation in the BMSCs treated with TNFα, BHI, or EfS were detected by luciferase assays with NFκBluc reporter. The TNFα group was used as the positive control. b Promotion of the NFκB activity by miR-200a-3p transfection. Luciferase assays were used to detect the NFκB activation of BMSCs after transfection with miR-200a-3p mimics or inhibitors. c The expression of p-NFκB was increased in the nucleus of BMSCs with transfection of miR-200a-3p. d NFκB and p-NFκB levels were detected by western blot in BMSCs treated with EfS together with miR-200a-3p mimics or inhibitors. Quantification of p-NFκB levels was calculated by normalizing with β-actin. e The migration of BMSCs could be attenuated by PDTC under the stimulation of EfS. DMSO was taken as the negative control. f Western blot analysis showed the effect of miR-200a-3p on p-NFκB could be repressed by PDTC. Quantifications of p-NFκB level were calculated by normalizing with β-actin. g The expression level of MMP-3 increased by miR-200a-3p could be attenuated by PDTC. h The expression level of MMP-13 increased by miR-200a-3p could be attenuated by PDTC. In g and h, quantification was calculated by normalizing with β-actin. i Schematic illustration implying the putative signaling pathways involved in the migration of BMSCs induced by E. faecalis supernatants. In response to E. faecalis supernatants, the transcription of miR-200a-3p is increased. Consequently, the expression of FOXJ1 is attenuated and its inhibition on nuclear factor kappa B (NFκB) pathway is abrogated. Activation and nucleus translocation of NFκB promotes the expressions of MMP-3 and MMP-13, which lead to the increased migration of BMSCs. *p < 0.05; NS, not significant. In c, bar = 50 μm